Part:BBa_K2239014

GDH (glucose dehydrogenas)

GDH (glucose dehydrogenase) catalyzes the conversion of beta-D-glucose to D-glucono-1,5-lactone and back, as it converts NADP+ to NADPH and back. It is used to catalyze the oxidation of beta-D-glucose into D-glucono-1,5-lactone and reduce NADP+ into NADPH during the process.[1]

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Information contributed by NFLS_Nanjing (2022)

Part information is collated here to help future users of the BioBrick registry.

Metadata:

• Group: NFLS_Nanjing (2022)
• Author: Yunshu Wang, Ziyi Ding, Qingying Deng
• Summary: Added information collated from existing scientific studies

Experimental approach:

1. Amplify the gdh gene by nested PCR with amplification system of 25μL, 10×buffer 2.5μL, dNTP 5nmol, primers 25 pmol each, ddH2O 17.3 μL, template DNA 2μL, Ex Taq 0.2U. The condition of the reaction is 94℃ for 5 min, 94℃ for 30s, 45℃ for 30s, 72℃ for 50s for 35 cycles, then 72℃ for 7 min.

2. The primers used in the first reaction is GDHeF and GDHiR, and the primers used in the second reaction is GDHiF and GDHiR.

3. The product of PCR is then tested by DNA agarose gel electrophoresis.

References

• Ghiffary M R , Prabowo C , Sharma K , et al. High-Level Production of the Natural Blue Pigment Indigoidine from Metabolically Engineered Corynebacterium glutamicum for Sustainable Fabric Dyes[J]. ACS Sustainable Chemistry And Engineering, 2021.
• Staiano M, Pennacchio A, Varriale A, Capo A, Majoli A, Capacchione C, D'Auria S. Enzymes as Sensors[J]. Methods in Enzymology, 2017